Rerio is comprised of "research release" basecalling models and configuration files. The research models provide cutting-edge functions, speeds and accuracies that have not been productionised or …

Basecalling models are frequently updated to improve accuracy and performance. The model version is identified using the following form v{major}.{minor}.{patch} for example v4.3.0. Simplex basecalling …

Basecalling using Guppy Basecalling using Guppy Base calling is the process of translating the electronic raw signal of the sequencer into bases, i.e., ATCG. As for most bioinformatic tasks there …

Automatic Basecaller Detection Detection Mechanism The workflow automatically extracts basecaller configuration metadata from input FASTQ or BAM files. This metadata is embedded during the …

Basecalling The basecaller translates the raw electrical signal from the sequencer into a nucleotide sequence in fastq format. As input the fast5 files as provided by the storage module are required. In …

Helper workflow for basecalling nanopore sequencing data. These are instructions to install and run the workflow on command line. You can also access the workflow via the EPI2ME Desktop application. …

2. Choosing a model To select the relevant model see the dorado repository for a table of available models to select for --basecaller_cfg and --remora_cfg. 3. Aligning to a reference The workflow can …

In this case we are using the “fast” model (rather than high-accuracy (hac) or super-high accuracy (sup)) to save time, and dna denotes that we sequenced…

Oct 26, 2023 · R10.4/Kit12 combined with sup basecalling holds promise as a singular sequencing technology in the reconstruction of commonly studied bacterial genomes, but hybrid assembly …

Workflow The following is a full DNAscent workflow, where we’ll start off after Dorado has finished running. The recommended Dorado basecalling model for v4.0.3 is …