Shotgun sequencing is performed by randomly fragmenting DNA sequences into small pieces, followed by computer-based reassembly of DNA fragments.

With the increased sequencing throughput and cost reductions, long-read full transcriptome sequencing is now being performed, either to generate highly accurate annotations for newly assembled ...

The novel DNA nanotechnology-driven method known as Light-seq allows full-transcriptome sequencing of hard-to-access cells in intact tissues.

RNA sequencing has emerged as a powerful supplement to DNA sequencing for Mendelian disease diagnosis, but clinical translation of diagnostic RNA-seq has not been widely achieved. Researchers at ...

Transcriptome (RNA sequencing) provides useful clinical information for treatment decision in the vast majority of pretreated cancer patients. This is an ASCO Meeting Abstract from the 2021 ASCO ...

Patients were assigned alterations on the basis of rates observed in published literature. Treatment approaches included no genomic testing; sequential single-gene testing; or a whole-exome, ...

This paper reports a valuable discovery that specific-mode electroacupuncture (EA) transiently opens the blood-brain barrier (BBB) in rats. The evidence is solid but lacks functional validation of BBB ...