Here we describe a simplified method for direct breakage of the emulsion using the DNA binding buffer (containing guanidine hydrochloride and isopropanol) that is provided with commonly used silica ...
The Roche/454 GS FLX System is based on emulsion PCR [8] and pyrophosphate detection [9] techniques. A library of DNA templates is constructed by a highly efficient DNA amplification method known as ...
The improvement of proteins and pathways for biotechnology and biomedical applications can be achieved by directed evolution 1,2. Directed evolution typically uses iterative rounds of selection or ...
Not quite 30 years old, the polymerase chain reaction (PCR) is the workhorse of many biology labs. While the basic premise—the amplification of DNA samples of interest—has remained constant, creative ...
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